Population structure of striped marlin (Kajikia audax) in the Pacific Ocean based on analysis of microsatellite and mitochondrial DNA

نویسندگان

  • Jan R. McDowell
  • John E. Graves
چکیده

Genetic variation was surveyed at five microsatellite loci and the mitochondrial control region (819 bp) to test for the presence of genetic stock structure in striped marlin (Kajikia audax) collections taken from seven locations throughout the Pacific Ocean. Temporal replicates separated by 9 years were taken off Japan, and three temporal samples spanning 11 years were collected off the coast of eastern Australia. Analyses of multilocus microsatellite genotypes and mitochondrial control region sequences showed no significant heterogeneity among collections taken from the same location in different years; however, significant spatial genetic heterogeneity was observed across all samples for microsatellite markers (FST = 0.013, P < 0.001). Mitochondrial control region sequences were not different across all samples ( ST = –0.01, P = 0.642). Analyses of molecular variance (AMOVA) revealed significant genetic differentiation between geographic regions for both microsatellite and mitochondrial markers. These results suggest the presence of genetically discrete populations within the Pacific Ocean and are supported by both the results of tagging studies, which show limited dispersal, and the presence of geographically separated spawning grounds. Résumé : Nous avons analysé la variation génétique à cinq locus microsatellites et dans la région mitochondriale de contrôle (819 pb) pour vérifier la présence de structure génétique dans des échantillons de stocks de marlins rayés (Kajikia audax) provenant de sept sites répartis dans tout le Pacifique. Nous avons pris des échantillons temporels à un intervalle de 9 années au large du Japon et trois échantillons temporels sur une période de 11 ans au large de la cōte de l’Australie orientale. Des analyses des génotypes à locus microsatellites multiples et des séquences de la région mitochondriale de contrôle n’indiquent aucune hétérogénéité significative entre les récoltes prises au même site durant des années différentes; on observe, cependant, une hétérogénéité génétique spatiale significative parmi l’ensemble des échantillons chez les marqueurs microsatellites (FST = 0,013, P < 0,001). Les séquences des régions mitochondriales de contrôle ne diffèrent pas dans l’ensemble des échantillons ( ST = –0,01, P = 0,642). Des analyses de la variance moléculaire (AMOVA) montrent une différenciation génétique significative entre les régions géographiques, tant chez les marqueurs microsatellites que mitochondriaux. Ces résultats font croire à l’existence de populations génétiquement distinctes au sein du Pacifique, ce qui est corroboré à la fois par les résultats des études de marquage qui révèlent une dispersion limitée et par la présence de sites de fraie séparés géographiquement. [Traduit par la Rédaction]

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تاریخ انتشار 2008